1. Objective Lens Selection
mm
NA
2. Tube Lens Selection
mm
mm
3. Digital Camera Sensor Selection
mm
mm
µm
µm
Magnification (M)
2.22×
fTL / fobj
Field of View
7.34 mm²
3.32 × 2.21 mm
Interrogated Vol.
0.734 µL
147% of 0.50 µL Target
Depth of Field
63.8 µm
Axial Focus Range
Optical Train Spatial Zones & Apertures:
Objective
RMS Mount
Exit Pupil:
Ø 9.0 mm
Ø 9.0 mm
Space 1: Infinity Space (L)
Collimated Parallel Rays
Houses Filter Cube & Dichroic
Max: 152.4 mm (No Vignetting)
Houses Filter Cube & Dichroic
Max: 152.4 mm (No Vignetting)
Tube Lens
Doublet
Clear Aperture:
Ø 22.5 mm
Ø 22.5 mm
Space 2: Mechanical Barrel
Converging Focused Rays
Physical Metal Extension Tube
Length: 82.5 mm (C-Mount FFD)
Physical Metal Extension Tube
Length: 82.5 mm (C-Mount FFD)
Camera
C-Mount (17.5mm in)
Sensor Diag:
Ø 8.9 mm
Ø 8.9 mm
| Parameter | Calculated Value | Assessment |
|---|---|---|
| Effective Object-Plane Scale: | 1.080 µm / pixel | Optimal |
| Linear Sampling Density: | 926 pixels / mm | High |
| Sampling Across 10 µm Stem Cell: | 9.26 pixels | Pass (≥ 5 px) |
| Rayleigh Optical Resolution Limit (λ = 530 nm): | 3.23 µm | Diffraction Limit |
| Nyquist Sampling Criterion (2 × r / Scale): | 5.99 (Over-sampled) | Valid (> 2.0) |
| Objective Exit Pupil Aperture (Øpupil = 2 × fobj × NA): | 9.00 mm | Beam Dia. |
| Tube Lens Clear Aperture (ØTL): | 22.50 mm | Lens Optic |
| Camera Sensor Diagonal Image Field (Øsensor = √(W² + H²)): | 8.86 mm | Image Circle |
|
Infinity Space (Space 1): Objective ➔ Tube Lens Max allowable distance for filter cube before vignetting: (ØTL − Øpupil) × fTL / Øsensor |
152.37 mm | Parallel Path |
|
Mechanical Barrel Length (Space 2): Tube Lens ➔ Camera Flange Physical metal extension tube length (fTL − 17.526 mm C-mount flange focal distance) |
82.47 mm | Focused Path |
Live Field of View Visualizer (Scale: 1 mm Grid)
3.32 mm × 2.21 mm
Governing Optical Formulas:
Magnification: M = fTL / fobj |
FOV Area: (Wsensor · Hsensor) / M² |
Rayleigh: r = 0.61λ / NA |
DoF: (λ / NA²) + (e / (M · NA)) |
Max Infinity Space: Lmax = (ØTL − 2·fobj·NA) · fTL / Øsensor |
Mechanical Barrel: fTL − 17.526 mm (C-Mount FFD)